Abstract

Corynebacterium glutamicum ATCC 13032 is a Gram-positive facultative anaerobic bacterium, which is a microbial workhorse in industrial biotechnology for the production of amino acids, in particular, glutamic acid. Due to its “GRAS” status, robustness and metabolic versatility, its use has been extended to produce fuels and various value-added products through metabolic engineering. In metabolic engineering, metabolic models such as constraint-based genome scale models (GSMs) and kinetic models (KMs) serve as valuable tools as they provide a mathematical representation of metabolic processes that in turn guide the rational design of genetic modifications to optimize production of desired compounds in the microbe. While GSMs emphasis on steady-state flux distribution, KMs enable a comprehensive dynamic visualization of the metabolic network, by simulating the rate of change of metabolite concentration. Though a GSM of C. glutamicum ATCC 13032 exists, there has been no whole cell KM to date. Here, we present a KM of C. glutamicum ATCC 13032 constructed using ab initio approach by identifying enzymes from its published genome. The resulting kinetic model, cglPS26, comprise of 986 metabolites, 443 enzymes with corresponding transcriptions and translations and 1153 enzymatic reactions. This can be a baseline model for incorporating additional cellular and growth processes, or as a system to examine cellular resource allocations necessary to guide engineering.

Keywords: Whole-cell model; Kinetic model; GRAS; Differential equations; AdvanceSyn Toolkit

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Citation

Maurice HT Ling., et al. “Ab Initio Whole Cell Kinetic Model of Corynebacterium glutamicum ATCC 13032 (cglPS26)". Clareus Scientific Medical Sciences 3.1 (2026): 21-26.

Copyright

© 2026 Maurice HT Ling., et al. Licensee Clareus Scientific Publications. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.