Leesha Haarshiny Perumal, Atoshi Abirami RajKumar, Cheryl Kai Ning Kang1,2, Sragvi Verma, Diya Nanthakumarvani, Shafeeqa Abul-Hasan and Maurice HT Ling*
Streptococcus pneumoniae is a pathogen able to utilize various carbon sources to produce polysaccharide capsule to avoid the immune system, leading to responsible for many life-threatening infections. Kinetic models may be used to examine S. pneumoniae’s carbon utilization but there are no whole-cell kinetic models of S. pneumoniae to date. In this study, we construct a whole-cell kinetic model based on S. pneumoniae NCTC 7465 using its annotated genome. The resulting model, spnLHP26, consists of 460 enzymes catalysing 836 reactions involving 163 metabolites; which may be suitable as a baseline draft model to examine virulence-associated metabolic susceptibilities of S. pneumoniae.
